Microbiology Unknown Project

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Sheet1

Gram Positive Chart
B Cereus B megaterium B subtilis E faecalis L lactis M luteus M roseus M phlei S ureae S aureus S epiderm S pyogenes S salivarius
Morphology rod>3um rod rod>3um coccus coccus coccus coccus rod coccus coccus coccus coccus coccus Morphology
Arrangement pairs,chains pairs,chains pairs,chains pairs,chains pairs,chains tetrads/clusters tetrads/clusters mycelium-like pairs,tetrads irreg clusters irreg clusters pairs,chains pairs,chains Arrangement
Pigments - - - - - Yellow Red - - - - - - Pigments
Motility + + + - - - - - + - - - - Motility
Starch [+] + + + - - [-] - [-] [-] d d Starch
Catalase + + + d - + + + + + + - - Catalase
Oxidase [+] - - - - + d + - - - - Oxidase
Indole - - - - - - - - - - - - Indole
VP + - + + + d d - + + + + VP
Citrate [+] + [+] [-] - + - + - d [-] - - Citrate
Urease [-] [+] - - - d - + + + - [+] Urease
Gelatinase + + + d - [+] [-] - + [+] - - Gelatinase
Glucose + + + + + [-] + - + + + + Glucose
Lactose - d [-] + + - - - + + + + Lactose
Sucrose + + + + + [-] + - + + + + Sucrose
Gas - - - - - - - - - - - - Gas
H2S - - - - - - - - - - - - H2S
Thioglycollate(o/f) Thioglycollate(o/f)
gram stain purple/blue purple/blue purple/blue purple/blue purple/blue purple/blue purple/blue purple/blue purple/blue purple/blue purple/blue purple/blue purple/blue gram stain
Morphology rod bacillus rod bacillus rod bacillus spherical coccus rod bacillus spherical coccus spherical coccus spherical coccus spherical coccus spherical coccus spherical coccus spherical coccus spherical coccus Morphology
arrangement CHAINS, STREPTO CHAINS, STREPTO CHAINS, STREPTO clusters, staphylo CHAINS, STREPTO TETRADS TETRADS TETRADS TETRADS, sarcinae clusters, staphylo clusters, staphylo CHAINS, STREPTO CHAINS, STREPTO arrangement
pigment - - - - - Yellow red - - - - - - pigment
colony morphology flat undulate translucent FLAT, IRREGULAR ROUND, SMOOTH CONVEX MUCOID MUCOID, LOBATE ROUND, ENTIRE,SHINY, CONVEX SMOOTH, CONVEX, ENTIRE SMOOTH, CONVEX, ENTIRE RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE colony morphology
growth in slant flat undulate translucent FLAT, IRREGULAR ROUND, SMOOTH CONVEX MUCOID MUCOID, LOBATE ROUND, ENTIRE,SHINY, CONVEX SMOOTH, CONVEX, ENTIRE SMOOTH, CONVEX, ENTIRE RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE growth in slant
growth in broth SEDIMENT SEDIMENT SEDIMENT UFT UFT SEDIMENT PELLICLE PELLICLE UFT UFT SEDIMENT SEDIMENT UFT growth in broth
sulfur (SIM) - - - - - - - - - - - - - sulfur (SIM)
indole (SIM) - - - - - - - - - - - - - indole (SIM)
motility (SIM) + + + - - + - - + - - - - motility (SIM)
catalase + + + ± - + + + + + + - - catalase
oxidase + - - - - + ± ± + - - - - oxidase
Methyl Red (MR) + - - + ± - ± - - - ± - - Methyl Red (MR)
vogues proskeur (VP) + - + + + - ± ± - + + + + vogues proskeur (VP)
citrate - + ± ± - + - + - ± ± - - citrate
Urease - ± - - - + - + + + + - ± Urease
Gelatinase - + + ± - - ± - - + ± - - Gelatinase
phenyl red dextrose + + + + + - + + - + - + + phenyl red dextrose
phenyl red lactose - - - + + - - - - + - + + phenyl red lactose
phenyl red sucrose + + + + + - + + - + - + + phenyl red sucrose
gas - - - - - - - - - - - - - gas
fluid thiolglycollate OA OA OA FA FA OA OA OA OA FA FA FA FA fluid thiolglycollate
Phenylalanine - - - - - - - + + + - + - Phenylalanine
starch agar plate + + + + - - ± + + ± ± ± ± starch agar plate
Casein(skim milk) - + + + ± - ± + + + - + - Casein(skim milk)
Lipase (TBA plate) - ± ± ± ± - ± - - - - - ± Lipase (TBA plate)
FA facultative anaerobe
OA obligate aerobe
+ 90% or greater positive
- 90% or greater negative
± 50% POSITIVE 50% NEGATIVE
UFT uniform fine turbidity

Sheet2

Gram Negative Chart
A hydrophila A faecalis C freundii E aerog E cloacae E coli K pneum P mirabilis P vulgaris P aeruginosa P fluorescens S marcescens S flexneri
gram stain red/pink red/pink red/pink red/pink red/pink red/pink red/pink red/pink red/pink red/pink red/pink red/pink red/pink gram stain
Morphology rod bacillus rod bacillus rod bacillus rod bacillus rod bacillus rod bacillus rod bacillus rod bacillus rod bacillus rod bacillus rod bacillus rod bacillus rod bacillus Morphology
arrangement SINGLE SINGLE SINGLE SINGLE SINGLE SINGLE SINGLE SINGLE SINGLE SINGLE SINGLE SINGLE SINGLE arrangement
pigment - - - - - - - - - blue/green yellow reddish orange - pigment
colony morphology FLAT/SMOOTH FLAT/SMOOTH MUCOID, SMOOTH MUCOID, convex MUCOID, convex FLAT, irregular, undulate, shiny MUCOID, SMOOTH MUCOID, SMOOTH MUCOID, SMOOTH FLAT/SMOOTH FLAT/SMOOTH MUCOID, convex MUCOID, SMOOTH colony morphology
growth in slant FLAT/SMOOTH FLAT/SMOOTH MUCOID, SMOOTH MUCOID, convex MUCOID, convex FLAT, irregular, undulate, shiny MUCOID, SMOOTH MUCOID, SMOOTH MUCOID, SMOOTH FLAT/SMOOTH FLAT/SMOOTH MUCOID, convex MUCOID, SMOOTH growth in slant
growth in broth SEDIMENT SEDIMENT SEDIMENT SEDIMENT SEDIMENT SEDIMENT SEDIMENT SEDIMENT SEDIMENT SEDIMENT SEDIMENT SEDIMENT SEDIMENT growth in broth
sulfur (SIM) ± - + - - - - ± + - - - - sulfur (SIM)
indole (SIM) + - - - - + - - + - - - ± indole (SIM)
motility (SIM) + + + + + + - + + + + + - motility (SIM)
catalase + + + + + + + + + + + + + catalase
oxidase + + - + - + - - - + + - - oxidase
Methyl Red ± - + - ± + ± + + - - - + Methyl Red
vogues proskeur (VP) ± - - - ± - - ± - - - + -
citrate ± + + + + - + ± ± + + + - citrate
Urease - - ± - ± - + + + ± ± ± - Urease
Gelatinase + - - - - - - + ± ± ± + - Gelatinase
phenyl red dextrose + - + + + + + + + - ± + + phenyl red dextrose
phenyl red lactose ± - + + ± + + - - - - - - phenyl red lactose
phenyl red sucrose + - ± + + - + - + - ± + - phenyl red sucrose
gas ± - + + + + + + + - - ± - gas
fluid thiolglycollate FA OA FA FA FA FA FA FA FA OA OA FA FA fluid thiolglycollate
Phenylalaine - - - - - - - + + - - - - Phenylalaine
starch agar plate + - - - - + + - - - - - - starch agar plate
casein(skim milk) - - - - - - - + + + + - - casein(skim milk)
lipids (TBA plate) - - - + + - + + + + + + - lipids (TBA plate)
FA facultative anaerobe
OA obligate aerobe
+ 90% or greater positive
- 90% or greater negative
± 50% POSITIVE 50% NEGATIVE
UFT uniform fine turbidity