Clinical genetics assignment
Clinical Genetics Module 10B Name_______ Homework problem 1
You are working with a circular plasmid that is 10.5 kb. When you digest it with BamHI and HindIII you get the following fragments.
Draw a restriction map that fits the data. Just draw it and take a photo
| Enzymes | Fragment sizes (in kb |
| BamHI | 7.3, 3.2 |
| HindIII | 5.1, 3.4, 2.0 |
| BamHI + HindIII | 4.6, 2.7, 2.0, 0.7, 0.5 |
Homework problem 2
A linear DNA is cut with 2 restriction enzymes, and these are the results. What is the order of the fragments? Draw the restriction map with the enzymes marked.
| Enzymes | Fragment sizes (in kb |
| Eco RI | 2.9, 4.5, 7.4, 8.0 |
| HindIII | 3.9, 6.0, 12.9 |
| Eco RI+ HindIII | 1.0, 2.0, 2.9, 3.5, 6.0, 7.4 |