biology

Care Hope College Microbiology-Lab Chap 9 Instructor: Dr. Gesner Cesar. Name: __________________________________________. Date: __________________________ 1-Definition: Biotechnology, Recombinant DNA (rDNA) technology, Vector, Clone, Complementary DNA (cDNA),synthetic DNA __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ _________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ 2-Definiition and function Polymerase Chain reaction, Electroporation, Transformation, Protoplast fusion __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________

3-Label the the typical genetic modification procedure

4-Label the different components of genomic libraries

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5-Recombination bacteria Blue-white screening, one method of selecting recombinant bacteria (Summarize the steps)

6-Definition: Random shotgun sequencing, bioinformatics, proteomics, nanotechnology, Ti Plasmid. __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ _________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________

__________________________________________________________________________________________________ __________________________________________________________________________________________________ 7-Difference and use of the following techniques: Southern blotting, DNA fingerprinting, Shotgun sequencing ________________________________________________________________________________________ ________________________________________________________________________________________ ______________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ _________________________________________________________________________________________________