Biology lab

Ezyme_concentration

Time		Absorbance
	1/2 X	1 X	2 X
0	0	0	0
20	0.011	0.023	0.05
40	0.024	0.05	0.1
60	0.068	0.16	0.3
80	0.08	0.17	0.34
100	0.1	0.3	0.63
120	0.200	0.4	0.82
Relative Enzyme Concentration	Reaction Rate
0	0
1/2 X	0.0013
1 X	0.0028
2 X	0.0058

Peroxidase Reactions Rates at different Enzyme Concentrations

0 1/2 X 1 X 2 X 0 1.2999999999999999E-3 2.8E-3 5.7999999999999996E-3

Relative Concentrations of Peroxidase

Reaction Rate (Absorbance units/sec)

The Effect of the enzyme Concentration on the Peroxidase Activity

1/2 X 0 20 40 60 80 100 120 0 1.0999999999999999E-2 2.4E-2 6.8000000000000005E-2 0.08 0.1 0.2 1 X 0 20 40 60 80 100 120 0 2. 3E-2 0.05 0.16 0.17 0.3 0.4 2 X

y = 0.0058x

0 20 40 60 80 100 120 0 0.05 0.1 0.3 0.34 0.63 0.82

Time (sec)

Absorbance

Temperatures

			Absorbance
Time
	0	23	40	60	100	Temperature (c)
0	0	0	0	0	0
20	0.07	0.12	0.11	0.09	0.003
40	0.18	0.4	0.3	0.27	0.004
60	0.21	0.52	0.4	0.35	0.002
80	0.37	0.72	0.64	0.52	0.001
100	0.46	0.83	0.72	0.63	0.001
120	0.51	0.94	0.8	0.73	0.001
Temperature	Reaction Rate
0	0.005
23	0.0083
40	0.0071
60	0.0062
100	0

The effect of different Temperature on the Peroxidase Activity

0 0 20 40 60 80 100 120 0 7.0000000000000007E-2 0.18 0.21 0.37 0.46 0.51 23 0 20 40 60 80 100 120 0 0.12 0.4 0.52 0.72 0.83 0.94 40 0 20 40 60 80 100 120 0 0.11 0.3 0.4 0.64 0.72 0.8 60 0 20 40 60 80 100 120 0 0.09 0.27 0.35 0.52 0.63 0.73 100 0 20 40 60 80 100 120 0 3.0000000000000001E-3 4.0000000000000001E-3 2E-3 1E-3 1E-3 1E-3

Temperature (degre C)

Absorbance

Effect of the different Temperatures on the Rates of the Peroxidase

0 23 40 60 5.0000000000000001E-3 8.3000000000000001E-3 7.1000000000000004E-3 6.1999999999999998E-3

pH of the solution

Reaction Rate (absorbance units/sec)

pH

Time		Absorbance
	pH3	pH5	pH7	pH9
0	0	0	0	0
20	0.015	0.2	0.15	0.001
40	0.025	0.3	0.2	0.001
60	0.025	0.33	0.22	0.001
80	0.025	0.42	0.25	0.001
100	0.035	0.56	0.31	0.001
120	0.036	0.58	0.25	0.001
Temperature	Reaction Rate
0	0.005
23	0.0083
40	0.0071
60	0.0062
100	0

Effect of the different pH on the Reaction Rates of the Peroxidase

0 23 40 60 5.0000000000000001E-3 8.3000000000000001E-3 7.1000000000000004E-3 6.1999999999999998E-3

pH of the solution

Reaction Rate (absorbance units/sec)

Effect of the pH on the Peroxidase Activity

pH3 0 20 40 60 80 100 120 0 1.4999999999999999E-2 2.5000000000000001E-2 2.5000000000000001E-2 2.5000000000000001E-2 3.5000000000000003E-2 3.5999999999999997E-2 pH5 0 20 40 60 80 100 120 0 0.2 0.3 0.33 0.42 0.56000000000000005 0.57999999999999996 pH7 0 20 40 60 80 100 120 0 0.15 0.2 0.22 0.25 0.31 0.25 pH9 0 20 40 60 80 100 120 0 1E-3 1E-3 1E-3 1E-3 1E-3 1E-3

Time (sec)

Absorbance

Proxidase_inhibitor

Time		Absorbance
	enzyme	inhibitor
0	0	0
20	0.022	0
40	0.05	0
60	0.14	0
80	0.18	0
100	0.31	0
120	0.42	0
	Reaction Rate
Enzyme	0.0029
Inhibitor	0

The effect of Hydroxylamine (an inhibitor) on the Peroxidase Activity

enzyme 0 20 40 60 80 100 120 0 2.1999999999999999E-2 0.05 0.14000000000000001 0.18 0.31 0.42 inhibitor 0 20 40 60 80 100 120 0 0 0 0 0 0 0

Time (sec)

Absorbance

Effect of an Inhibitor on Peroxidase Activity

Enzyme Inhibitor 2.8999999999999998E-3 0

Reaction Rate (Absorbance units/sec)