BIO 151 Lab

Name__________________________ BIOL 151

Lab Assignment: Karyotyping

Instructions:

Refer to Chapter 10 and Chapter 13 in the textbook and the file “Karyotyping Background

Information” for this lab activity.

Karyotyping Exercise

Pre-lab questions.

Answer the following questions in your lab notebooks. Not everything will be found in your

textbook. Use the internet to help you find the answers:

1. What chromosomal change(s) is/are the cause of each of the following human conditions?

a. Down’s Syndrome: b. Edwards Syndrome: c. Patau Syndrome:

2. Which of the human chromosomes is the longest?

3. Of the two human sex chromosomes, which is longer, the X or the Y?

4. In what phase of cell division are cells arrested in order to perform a chromosome spread

and karyotype?

Procedure:

Record your work in your lab notebook:

1. Date and title of the exercise 2. Purpose 3. Procedure (cite the website) 4. Results

a. Take a screen shot of each completed karyotype (there will be three). b. Use each image to make a figure. c. Include Figure # and a descriptive title for each image.

5. Discussion a. A few sentences about your conclusions. b. What are your diagnoses for Patients A, B, and C?

Click on the link provided, and complete the karyotyping exercise. Read the introductory

information, and then click the “patient histories” button at the bottom of the screen. Then finish

each patient’s karyotype by clicking on the patient links and double clicking on the location where

each given chromosome should be placed. Take a screen shot of each completed karyotype. These

images will become figures for your lab assignment.

Karyotyping Activity

Post-Activity Questions

1. What diagnosis (Klinefelter, for example) would you give patient A?

2. What diagnosis (Klinefelter, for example) would you give patient B?

3. What diagnosis (Klinefelter, for example) would you give patient C?