genetics problems
Hong BIOL 360 Sp 2021
Genetics Pre-Exam 3 Questions
Useful resources:
https://web.expasy.org/translate/
https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&BLAST_SPEC=blast2seq&
LINK_LOC=align2seq (BLAST2 to compare any two sequences)
https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins (BLAST search DNA or protein
against databases)
www.benchling.com (DNA editor and restriction sites)
Hong BIOL 360 Sp 2021
Exam 3 DNA problems: Work on these problems BEFORE the exam. DO NOT discuss these problems with anyone, as you are expected to work on them on your own. The actual exam questions will require you to have some of these tanswers on hand and document your work. 1. Predict a possible phenotype from the following mutations in the Lac Operon: a deletion in “GGAATTGTGAGCGGATAACAATTTCAC.” 2. Using the BLASTP search, find out which is the closest biological source for this protein? (https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins). How is this protein related to one you have worked on before? How closely related are the new protein and the ‘old’ protein in percentage of identity?
>protein Y
MIHSVFLLMFLLTPTESYVDVGPDSVKSACIEVDIQQTFFDKTWPRPIDVSKADGIIYPQ
GRTYSNITITYQGLFPYQGDHGDMYVYSAGHATGTTPQKLFVANYSQDVKQFANGFVVRI
GAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDGKMGRFFNHTLVLLPDGCGTLL
RAFYCILEPRSGNHCPAGNSHTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFM
YTYNITEDEILEWFGITQTAQGVHLFSSRYVDLYGGNMFQFATLPVYDTIKYYSIIPHSI
RSIQSDRKAWAAFYVYKLQPLTFLLDFSVDGYIRRAIDCGFNDLSQLHCSYESFDVESGV
YSVSSFEAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSV
NDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLI
LATVPHNLTTITKPLKYSYINKCSRLLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDY
YRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQL
GNCVEYSLYGVSGRGVFQNCTAVGVRQQRFVYDAYQNLVGYYSDDGNYYCLRACVSVPVS
VIYDKETKTHATLFGSVACEHISSTMSQYSRSTRSMLKRRDSTYGPLQTPVGCVLGLVNS
SLFVEDCKLPLGQSLCALPDTPSTLTPRSVRSVPGEMRLASIAFNHPIQVDQLNSSYFKL
SIPTNFSFGVTQEYIQTTIQKVTVDCKQYVCNGFQKCEQLLREYGQFCSKINQALHGANL
RQDDSVRNLFASVKSSQSSPIIPGFGGDFNLTLLEPVSISTGSRSARSAIEDLLFDKVTI
ADPGYMQGYDDCMQQGPASARDLICAQYVAGYKVLPPLMDVNMEAAYTSSLLGSIAGVGW
TAGLSSFAAIPFAQSIFYRLNGVGITQQVLSENQKLIANKFNQALGAMQTGFTTTNEAFR
KVQDAVNNNAQALSKLASELSNTFGAISASIGDIIQRLDVLEQDAQIDRLINGRLTTLNA
FVAQQLVRSESAALSAQLAKDKVNECVKAQSKRSGFCGQGTHIVSFVVNAPNGLYFMHVG
YYPSNHIEVVSAYGLCDAANPTNCIAPVNGYFIKTNNTRIVDEWSYTGSSFYAPEPITSL
NTKYVAPQVTYQNISTNLPPPLLGNSTGIDFQDELDEFFKNVSTSIPNFGSLTQINTTLL
DLTYEMLSLQQVVKALNESYIDLKELGNYTYYNKWPWYIWLGFIAGLVALALCVFFILCC
TGCGTNCMGKLKCNRCCDRYEEYDLEPHKVHVH
3. We will re-analyze our old sequence, the gene that can cause hemophilia in humans,
coagulating factor VIII. The mRNA sequence (as DNA) is shown below. What is the longest
open reading frame (ORF)?
4. What is the longest fragment from this gene you can clone using HindIII (A’AGCTT)?
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5. If you successfully cloned this longest fragment into pUC19, what are the two possible
restriction patterns on a gel you can expect using XbaI (T’CTAGA)?
>NM_000132.4|coagulating factor VIII
GCTTAGTGCTGAGCACATCCAGTGGGTAAAGTTCCTTAAAATGCTCTGCAAAGAAATTGGGACTTTTCAT
TAAATCAGAAATTTTACTTTTTTCCCCTCCTGGGAGCTAAAGATATTTTAGAGAAGAATTAACCTTTTGC
TTCTCCAGTTGAACATTTGTAGCAATAAGTCATGCAAATAGAGCTCTCCACCTGCTTCTTTCTGTGCCTT
TTGCGATTCTGCTTTAGTGCCACCAGAAGATACTACCTGGGTGCAGTGGAACTGTCATGGGACTATATGC
AAAGTGATCTCGGTGAGCTGCCTGTGGACGCAAGATTTCCTCCTAGAGTGCCAAAATCTTTTCCATTCAA
CACCTCAGTCGTGTACAAAAAGACTCTGTTTGTAGAATTCACGGATCACCTTTTCAACATCGCTAAGCCA
AGGCCACCCTGGATGGGTCTGCTAGGTCCTACCATCCAGGCTGAGGTTTATGATACAGTGGTCATTACAC
TTAAGAACATGGCTTCCCATCCTGTCAGTCTTCATGCTGTTGGTGTATCCTACTGGAAAGCTTCTGAGGG
AGCTGAATATGATGATCAGACCAGTCAAAGGGAGAAAGAAGATGATAAAGTCTTCCCTGGTGGAAGCCAT
ACATATGTCTGGCAGGTCCTGAAAGAGAATGGTCCAATGGCCTCTGACCCACTGTGCCTTACCTACTCAT
ATCTTTCTCATGTGGACCTGGTAAAAGACTTGAATTCAGGCCTCATTGGAGCCCTACTAGTATGTAGAGA
AGGGAGTCTGGCCAAGGAAAAGACACAGACCTTGCACAAATTTATACTACTTTTTGCTGTATTTGATGAA
GGGAAAAGTTGGCACTCAGAAACAAAGAACTCCTTGATGCAGGATAGGGATGCTGCATCTGCTCGGGCCT
GGCCTAAAATGCACACAGTCAATGGTTATGTAAACAGGTCTCTGCCAGGTCTGATTGGATGCCACAGGAA
ATCAGTCTATTGGCATGTGATTGGAATGGGCACCACTCCTGAAGTGCACTCAATATTCCTCGAAGGTCAC
ACATTTCTTGTGAGGAACCATCGCCAGGCGTCCTTGGAAATCTCGCCAATAACTTTCCTTACTGCTCAAA
CACTCTTGATGGACCTTGGACAGTTTCTACTGTTTTGTCATATCTCTTCCCACCAACATGATGGCATGGA
AGCTTATGTCAAAGTAGACAGCTGTCCAGAGGAACCCCAACTACGAATGAAAAATAATGAAGAAGCGGAA
GACTATGATGATGATCTTACTGATTCTGAAATGGATGTGGTCAGGTTTGATGATGACAACTCTCCTTCCT
TTATCCAAATTCGCTCAGTTGCCAAGAAGCATCCTAAAACTTGGGTACATTACATTGCTGCTGAAGAGGA
GGACTGGGACTATGCTCCCTTAGTCCTCGCCCCCGATGACAGAAGTTATAAAAGTCAATATTTGAACAAT
GGCCCTCAGCGGATTGGTAGGAAGTACAAAAAAGTCCGATTTATGGCATACACAGATGAAACCTTTAAGA
CTCGTGAAGCTATTCAGCATGAATCAGGAATCTTGGGACCTTTACTTTATGGGGAAGTTGGAGACACACT
GTTGATTATATTTAAGAATCAAGCAAGCAGACCATATAACATCTACCCTCACGGAATCACTGATGTCCGT
CCTTTGTATTCAAGGAGATTACCAAAAGGTGTAAAACATTTGAAGGATTTTCCAATTCTGCCAGGAGAAA
TATTCAAATATAAATGGACAGTGACTGTAGAAGATGGGCCAACTAAATCAGATCCTCGGTGCCTGACCCG
CTATTACTCTAGTTTCGTTAATATGGAGAGAGATCTAGCTTCAGGACTCATTGGCCCTCTCCTCATCTGC
TACAAAGAATCTGTAGATCAAAGAGGAAACCAGATAATGTCAGACAAGAGGAATGTCATCCTGTTTTCTG
TATTTGATGAGAACCGAAGCTGGTACCTCACAGAGAATATACAACGCTTTCTCCCCAATCCAGCTGGAGT
GCAGCTTGAGGATCCAGAGTTCCAAGCCTCCAACATCATGCACAGCATCAATGGCTATGTTTTTGATAGT
TTGCAGTTGTCAGTTTGTTTGCATGAGGTGGCATACTGGTACATTCTAAGCATTGGAGCACAGACTGACT
TCCTTTCTGTCTTCTTCTCTGGATATACCTTCAAACACAAAATGGTCTATGAAGACACACTCACCCTATT
CCCATTCTCAGGAGAAACTGTCTTCATGTCGATGGAAAACCCAGGTCTATGGATTCTGGGGTGCCACAAC
TCAGACTTTCGGAACAGAGGCATGACCGCCTTACTGAAGGTTTCTAGTTGTGACAAGAACACTGGTGATT
ATTACGAGGACAGTTATGAAGATATTTCAGCATACTTGCTGAGTAAAAACAATGCCATTGAACCAAGAAG
CTTCTCCCAGAATTCAAGACACCCTAGCACTAGGCAAAAGCAATTTAATGCCACCACAATTCCAGAAAAT
GACATAGAGAAGACTGACCCTTGGTTTGCACACAGAACACCTATGCCTAAAATACAAAATGTCTCCTCTA
GTGATTTGTTGATGCTCTTGCGACAGAGTCCTACTCCACATGGGCTATCCTTATCTGATCTCCAAGAAGC
CAAATATGAGACTTTTTCTGATGATCCATCACCTGGAGCAATAGACAGTAATAACAGCCTGTCTGAAATG
ACACACTTCAGGCCACAGCTCCATCACAGTGGGGACATGGTATTTACCCCTGAGTCAGGCCTCCAATTAA
GATTAAATGAGAAACTGGGGACAACTGCAGCAACAGAGTTGAAGAAACTTGATTTCAAAGTTTCTAGTAC
ATCAAATAATCTGATTTCAACAATTCCATCAGACAATTTGGCAGCAGGTACTGATAATACAAGTTCCTTA
GGACCCCCAAGTATGCCAGTTCATTATGATAGTCAATTAGATACCACTCTATTTGGCAAAAAGTCATCTC
CCCTTACTGAGTCTGGTGGACCTCTGAGCTTGAGTGAAGAAAATAATGATTCAAAGTTGTTAGAATCAGG
TTTAATGAATAGCCAAGAAAGTTCATGGGGAAAAAATGTATCGTCAACAGAGAGTGGTAGGTTATTTAAA
GGGAAAAGAGCTCATGGACCTGCTTTGTTGACTAAAGATAATGCCTTATTCAAAGTTAGCATCTCTTTGT
TAAAGACAAACAAAACTTCCAATAATTCAGCAACTAATAGAAAGACTCACATTGATGGCCCATCATTATT
AATTGAGAATAGTCCATCAGTCTGGCAAAATATATTAGAAAGTGACACTGAGTTTAAAAAAGTGACACCT
TTGATTCATGACAGAATGCTTATGGACAAAAATGCTACAGCTTTGAGGCTAAATCATATGTCAAATAAAA
CTACTTCATCAAAAAACATGGAAATGGTCCAACAGAAAAAAGAGGGCCCCATTCCACCAGATGCACAAAA
TCCAGATATGTCGTTCTTTAAGATGCTATTCTTGCCAGAATCAGCAAGGTGGATACAAAGGACTCATGGA
AAGAACTCTCTGAACTCTGGGCAAGGCCCCAGTCCAAAGCAATTAGTATCCTTAGGACCAGAAAAATCTG
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TGGAAGGTCAGAATTTCTTGTCTGAGAAAAACAAAGTGGTAGTAGGAAAGGGTGAATTTACAAAGGACGT
AGGACTCAAAGAGATGGTTTTTCCAAGCAGCAGAAACCTATTTCTTACTAACTTGGATAATTTACATGAA
AATAATACACACAATCAAGAAAAAAAAATTCAGGAAGAAATAGAAAAGAAGGAAACATTAATCCAAGAGA
ATGTAGTTTTGCCTCAGATACATACAGTGACTGGCACTAAGAATTTCATGAAGAACCTTTTCTTACTGAG
CACTAGGCAAAATGTAGAAGGTTCATATGACGGGGCATATGCTCCAGTACTTCAAGATTTTAGGTCATTA
AATGATTCAACAAATAGAACAAAGAAACACACAGCTCATTTCTCAAAAAAAGGGGAGGAAGAAAACTTGG
AAGGCTTGGGAAATCAAACCAAGCAAATTGTAGAGAAATATGCATGCACCACAAGGATATCTCCTAATAC
AAGCCAGCAGAATTTTGTCACGCAACGTAGTAAGAGAGCTTTGAAACAATTCAGACTCCCACTAGAAGAA
ACAGAACTTGAAAAAAGGATAATTGTGGATGACACCTCAACCCAGTGGTCCAAAAACATGAAACATTTGA
CCCCGAGCACCCTCACACAGATAGACTACAATGAGAAGGAGAAAGGGGCCATTACTCAGTCTCCCTTATC
AGATTGCCTTACGAGGAGTCATAGCATCCCTCAAGCAAATAGATCTCCATTACCCATTGCAAAGGTATCA
TCATTTCCATCTATTAGACCTATATATCTGACCAGGGTCCTATTCCAAGACAACTCTTCTCATCTTCCAG
CAGCATCTTATAGAAAGAAAGATTCTGGGGTCCAAGAAAGCAGTCATTTCTTACAAGGAGCCAAAAAAAA
TAACCTTTCTTTAGCCATTCTAACCTTGGAGATGACTGGTGATCAAAGAGAGGTTGGCTCCCTGGGGACA
AGTGCCACAAATTCAGTCACATACAAGAAAGTTGAGAACACTGTTCTCCCGAAACCAGACTTGCCCAAAA
CATCTGGCAAAGTTGAATTGCTTCCAAAAGTTCACATTTATCAGAAGGACCTATTCCCTACGGAAACTAG
CAATGGGTCTCCTGGCCATCTGGATCTCGTGGAAGGGAGCCTTCTTCAGGGAACAGAGGGAGCGATTAAG
TGGAATGAAGCAAACAGACCTGGAAAAGTTCCCTTTCTGAGAGTAGCAACAGAAAGCTCTGCAAAGACTC
CCTCCAAGCTATTGGATCCTCTTGCTTGGGATAACCACTATGGTACTCAGATACCAAAAGAAGAGTGGAA
ATCCCAAGAGAAGTCACCAGAAAAAACAGCTTTTAAGAAAAAGGATACCATTTTGTCCCTGAACGCTTGT
GAAAGCAATCATGCAATAGCAGCAATAAATGAGGGACAAAATAAGCCCGAAATAGAAGTCACCTGGGCAA
AGCAAGGTAGGACTGAAAGGCTGTGCTCTCAAAACCCACCAGTCTTGAAACGCCATCAACGGGAAATAAC
TCGTACTACTCTTCAGTCAGATCAAGAGGAAATTGACTATGATGATACCATATCAGTTGAAATGAAGAAG
GAAGATTTTGACATTTATGATGAGGATGAAAATCAGAGCCCCCGCAGCTTTCAAAAGAAAACACGACACT
ATTTTATTGCTGCAGTGGAGAGGCTCTGGGATTATGGGATGAGTAGCTCCCCACATGTTCTAAGAAACAG
GGCTCAGAGTGGCAGTGTCCCTCAGTTCAAGAAAGTTGTTTTCCAGGAATTTACTGATGGCTCCTTTACT
CAGCCCTTATACCGTGGAGAACTAAATGAACATTTGGGACTCCTGGGGCCATATATAAGAGCAGAAGTTG
AAGATAATATCATGGTAACTTTCAGAAATCAGGCCTCTCGTCCCTATTCCTTCTATTCTAGCCTTATTTC
TTATGAGGAAGATCAGAGGCAAGGAGCAGAACCTAGAAAAAACTTTGTCAAGCCTAATGAAACCAAAACT
TACTTTTGGAAAGTGCAACATCATATGGCACCCACTAAAGATGAGTTTGACTGCAAAGCCTGGGCTTATT
TCTCTGATGTTGACCTGGAAAAAGATGTGCACTCAGGCCTGATTGGACCCCTTCTGGTCTGCCACACTAA
CACACTGAACCCTGCTCATGGGAGACAAGTGACAGTACAGGAATTTGCTCTGTTTTTCACCATCTTTGAT
GAGACCAAAAGCTGGTACTTCACTGAAAATATGGAAAGAAACTGCAGGGCTCCCTGCAATATCCAGATGG
AAGATCCCACTTTTAAAGAGAATTATCGCTTCCATGCAATCAATGGCTACATAATGGATACACTACCTGG
CTTAGTAATGGCTCAGGATCAAAGGATTCGATGGTATCTGCTCAGCATGGGCAGCAATGAAAACATCCAT
TCTATTCATTTCAGTGGACATGTGTTCACTGTACGAAAAAAAGAGGAGTATAAAATGGCACTGTACAATC
TCTATCCAGGTGTTTTTGAGACAGTGGAAATGTTACCATCCAAAGCTGGAATTTGGCGGGTGGAATGCCT
TATTGGCGAGCATCTACATGCTGGGATGAGCACACTTTTTCTGGTGTACAGCAATAAGTGTCAGACTCCC
CTGGGAATGGCTTCTGGACACATTAGAGATTTTCAGATTACAGCTTCAGGACAATATGGACAGTGGGCCC
CAAAGCTGGCCAGACTTCATTATTCCGGATCAATCAATGCCTGGAGCACCAAGGAGCCCTTTTCTTGGAT
CAAGGTGGATCTGTTGGCACCAATGATTATTCACGGCATCAAGACCCAGGGTGCCCGTCAGAAGTTCTCC
AGCCTCTACATCTCTCAGTTTATCATCATGTATAGTCTTGATGGGAAGAAGTGGCAGACTTATCGAGGAA
ATTCCACTGGAACCTTAATGGTCTTCTTTGGCAATGTGGATTCATCTGGGATAAAACACAATATTTTTAA
CCCTCCAATTATTGCTCGATACATCCGTTTGCACCCAACTCATTATAGCATTCGCAGCACTCTTCGCATG
GAGTTGATGGGCTGTGATTTAAATAGTTGCAGCATGCCATTGGGAATGGAGAGTAAAGCAATATCAGATG
CACAGATTACTGCTTCATCCTACTTTACCAATATGTTTGCCACCTGGTCTCCTTCAAAAGCTCGACTTCA
CCTCCAAGGGAGGAGTAATGCCTGGAGACCTCAGGTGAATAATCCAAAAGAGTGGCTGCAAGTGGACTTC
CAGAAGACAATGAAAGTCACAGGAGTAACTACTCAGGGAGTAAAATCTCTGCTTACCAGCATGTATGTGA
AGGAGTTCCTCATCTCCAGCAGTCAAGATGGCCATCAGTGGACTCTCTTTTTTCAGAATGGCAAAGTAAA
GGTTTTTCAGGGAAATCAAGACTCCTTCACACCTGTGGTGAACTCTCTAGACCCACCGTTACTGACTCGC
TACCTTCGAATTCACCCCCAGAGTTGGGTGCACCAGATTGCCCTGAGGATGGAGGTTCTGGGCTGCGAGG
CACAGGACCTCTACTGAGGGTGGCCACTGCAGCACCTGCCACTGCCGTCACCTCTCCCTCCTCAGCTCCA
GGGCAGTGTCCCTCCCTGGCTTGCCTTCTACCTTTGTGCTAAATCCTAGCAGACACTGCCTTGAAGCCTC
CTGAATTAACTATCATCAGTCCTGCATTTCTTTGGTGGGGGGCCAGGAGGGTGCATCCAATTTAACTTAA
CTCTTACCTATTTTCTGCAGCTGCTCCCAGATTACTCCTTCCTTCCAATATAACTAGGCAAAAAGAAGTG
AGGAGAAACCTGCATGAAAGCATTCTTCCCTGAAAAGTTAGGCCTCTCAGAGTCACCACTTCCTCTGTTG
TAGAAAAACTATGTGATGAAACTTTGAAAAAGATATTTATGATGTTAACATTTCAGGTTAAGCCTCATAC
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GTTTAAAATAAAACTCTCAGTTGTTTATTATCCTGATCAAGCATGGAACAAAGCATGTTTCAGGATCAGA
TCAATACAATCTTGGAGTCAAAAGGCAAATCATTTGGACAATCTGCAAAATGGAGAGAATACAATAACTA
CTACAGTAAAGTCTGTTTCTGCTTCCTTACACATAGATATAATTATGTTATTTAGTCATTATGAGGGGCA
CATTCTTATCTCCAAAACTAGCATTCTTAAACTGAGAATTATAGATGGGGTTCAAGAATCCCTAAGTCCC
CTGAAATTATATAAGGCATTCTGTATAAATGCAAATGTGCATTTTTCTGACGAGTGTCCATAGATATAAA
GCCATTTGGTCTTAATTCTGACCAATAAAAAAATAAGTCAGGAGGATGCAATTGTTGAAAGCTTTGAAAT
AAAATAACAATGTCTTCTTGAAATTTGTGATGGCCAAGAAAGAAAATGATGATGACATTAGGCTTCTAAA
GGACATACATTTAATATTTCTGTGGAAATATGAGGAAAATCCATGGTTATCTGAGATAGGAGATACAAAC
TTTGTAATTCTAATAATGCACTCAGTTTACTCTCTCCCTCTACTAATTTCCTGCTGAAAATAACACAACA
AAAATGTAACAGGGGAAATTATATACCGTGACTGAAAACTAGAGTCCTACTTACATAGTTGAAATATCAA
GGAGGTCAGAAGAAAATTGGACTGGTGAAAACAGAAAAAACACTCCAGTCTGCCATATCACCACACAATA
GGATCCCCCTTCTTGCCCTCCACCCCCATAAGATTGTGAAGGGTTTACTGCTCCTTCCATCTGCCTGACC
CCTTCACTATGACTACACAGAATCTCCTGATAGTAAAGGGGGCTGGAGACAAGGATAAGTTATAGAGCAG
TTGGAGGAAGCATCCAAAGATTGCAACCCAGGGCAAATGGAAAACAGGAGATCCTAATATGAAAGAAAAA
TGGATCCCAATCTGAGAAAAGGCAAAAGAATGGCTACTTTTTTCTATGCTGGAGTATTTTCTAATAATCC
TGCTTGACCCTTATCTGACCTCTTTGGAAACTATAACATAGCTGTCACAGTATAGTCACAATCCACAAAT
GATGCAGGTGCAAATGGTTTATAGCCCTGTGAAGTTCTTAAAGTTTAGAGGCTAACTTACAGAAATGAAT
AAGTTGTTTTGTTTTATAGCCCGGTAGAGGAGTTAACCCCAAAGGTGATATGGTTTTATTTCCTGTTATG
TTTAACTTGATAATCTTATTTTGGCATTCTTTTCCCATTGACTATATACATCTCTATTTCTCAAATGTTC
ATGGAACTAGCTCTTTTATTTTCCTGCTGGTTTCTTCAGTAATGAGTTAAATAAAACATTGACACATACA
AA
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Problems 6-10: Consider the following sequences from a gene for nematode olfaction.
>odr-3 cDNA
ATGGGATTGTGTCAGAGCAGCTCAGAAGTTGATAAGGAACAACAGCAGCGAAATAAG
GAAATCGAAAAGCAGATTAACTCGGACAAACGATCAGCATCGAGTATCGTCAAACTT
CTTTTGTTAGGAGCTGGAGAATGCGGAAAATCGACAGTTTTGAAGCAGATGCAAATTC
TGCATTCCAATGGATTCACGGAAGAGGAAGTGAATGAGAAACGAGGAATCGTTTTCA
ATAACACGGTCACATCAATGTGTTCCATACTCCGAGCAATGGATGCTGTACTTCACAT
TCAATTAGAGAACTCCGAATTGGAGCCCGAGAAAGCTATGATTCTCAAGATTCAAGA
GAAGGGAGAAGAATCGGAACAGATGACACCTGAAAAACGAGATGCCCTGCTCGCATT
GTGGCAGGATAAAGGAGTGAAGAAAGCATATGAAATGAGGAGTGAATACCAACTCAT
CGACTCTGCTGCATTGTACGTTTCCCCTCTTCATACATCAAGATCTTCTATGATGCGTG
CTATTCCCTCCCTATCACTGCAGCCTAATGAGTACTTCCCCTTCAGTTTCCTCGATAAT
GTTCAACGCATCGCTGAGCCTGGATTCCGACCGACTGAGCAAGATATTCTCTTCTCGC
GAGTGGCCACAACCGGAGTCGTCGAAGTGAAATTCAAAATCAAGGAACTCGATTTCA
GAGTATTCGACGTAGGAGGGCAACGATCGGAGAGAAGAAAGTGGATTCACTGTTTCG
ATAACGTCGAATCAATCATTTTCATCACTGCCATCTCTGAATACGATCAAGTTCTTTTC
GAGGATGAAAGCACAAATCGTATGATCGAATCGATGCAACTCTTCAATTCCATCTGCA
ATTCTTCATGGTTTTTGTCTACTGCTATGATTCTCTTCATGAATAAGAAGGATCTCTTC
ATGGAGAAGATCAAACGCGTAAACATCACAACCGCCTTCCCTGATTATGAAGGAGGG
CAAAACTACGAAGAAGCAGTGAATTTCATCAAGCAAAAGTTCGCTGAACTAAATATG
AACCCTGATAAGAAGACGATTTACATGCACGAGACTTGCGCCACGGACACTAATCAG
GTTCAACTGGTCATTTCCTCTGTCATCGATACGATTATCCAGAAGAATCTACAGAAGG
CAGGAATGATGTAG
>odr-3 gDNA
ATGGGATTGTGTCAGAGCAGCTCAGAAGTTGATAAGGAACAACAGGTATTTCAATTAT
TCATCACATCAATCTCGTAATATCGTCGTCCCCATCTCTTCACCCACCCAAGTGCATTG
AATCTAATCATCCTCAATTTCAGCAGCGAAATAAGGAAATCGAAAAGCAGATTAACT
CGGACAAACGATCAGCATCGAGTATCGTCAAACTTCTTTTGTTAGGTAAGATCACAGA
TTGAAAGATCAAAGATCAATCCACTTATTTTCAGGAGCTGGAGAATGCGGAAAATCG
ACAGTTTTGAAGCAGATGCAGTGAGTTTTTGGGAACTTCAACTTTGAGCTTATGAGAG
AAGACAGATGTTTCGATCTGACAGAGAACAGAGGAGAACATTTTAGACATGAACTAT
TTCAGAATTCTGCATTCCAATGGATTCACGGAAGAGGAAGTGAATGAGAAACGAGGA
ATCGTTTTCAATAACACGGTGAGATTATCGATCAAGGGGATGTCATGACTAATCAATT
CATTACAGGTCACATCAATGTGTTCCATACTCCGAGCAATGGATGCTGTACTTCACATT
CAATTAGAGAACTCCGAATTGGAGGTCGGTTTATGATCTCCCTTTATCACACCTTCAA
ATAGTGTATGAATTGTATTGCAATCAAGGAACTGTTCCATGAACCTTTTTGTATCTCCC
ATTAAATTCATTGGGATCCGTGTTGATAGTGAATCTCAATCTCATGTGTATCGTGTAGT
CAGGGGCAACTGAGAAGGGGGGAAAGAAACGACGGGGTATTCATGAGATCCAGAAG
GGGATTGAGAGGGGGACTGAGTAGGAGTGAGAAGGGAGAGGGGGGAGGGGACGGG
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GACGGCTGTAATGGGAAAGATATAGGACGATCGACGTCAATAATCGTTGAAATTTGA
ACATGGGACCGACTCTGAAAAGGAACGATTTCAGCCCGAGAAAGCTATGATTCTCAA
GATTCAAGAGAAGGGAGAAGAATCGGAACAGATGACACCTGAAAAACGGTGAGTGA
CGACGATCGATTGATGATTCACCCATAACTGTGATTGCAGAGATGCCCTGCTCGCATT
GTGGCAGGATAAAGGAGTGAAGAAAGCATATGAAATGAGGAGTGAATACCAACTCAT
CGACTCTGCTGCATTGTACGTTTCCCCTCTTCATACATCAAGATCTTCTATGATGCGTG
CTATTCCCTCCCTATCACTGCAGCCTAATGAGTACTTCCCCTTCAGTTTCCTCGATAAT
GTTCAACGCATCGCTGAGCCTGGATTCCGACCGACTGAGCAAGATATTCTCTTCTCGC
GAGTGGCCACAACCGGAGTCGTCGAAGTGAAATTCAAAATCAAGGAACTCGATTTCA
GGTCGGAATTAATGATTGATGGAGGCTGTTTATAGGAAATTGTCTGGAGTTGCTTTTC
TTATCACTTGTTATTCCTTCGTTTTCCCATTTCTCGTCGTGTGAAGAAGGAGACGATAT
CGTCATAATGGATTAGTATCGATTGAGGGAGAGGGAAAGGGGGAGGGGGGAGGGGG
GGAGGCACTCGAAGAGAAGCTAATGAAGTCCACGGGAAGAAGGGATTTACACTAGA
AGAGACTGACTACAGAATGGAGGCTAGAGATTAAACTGATGAGAGATTATTATGAAT
ACAATTACAATAACACACAATTATGAGTATTCTTAGATTGGTATTGTGCATCAGACAT
CTAGGAAATGGATTAATACTCATTCAATGCTTGCATCTCTTCAGAGTATTCGACGTAG
GAGGGCAACGATCGGAGAGAAGAAAGTGGATTCACTGTTTCGATAACGTCGAATCAA
TCATTTTCATCACTGCCATCTCTGAATACGATCAAGTTCTTTTCGAGGATGAAAGCACA
GTGAGAATTTACCCCCTTTGAGATCAATACAGTTCGAAATTCGGGTGATCGTCTCTTC
ATTTCAAATGATCTCTCATTAGTCAGTGCGACGAGAGAGTGTGTAAAATGAAGAGAC
GATTGTTCGAAGGTCGACCTCTAGAGATCATCGATCATTATTGCGTATGAGACAACGA
GTCATACATTCAATTCAATTTGAACAGAAACAGACATCGAAAGCACTTGAGGCACTTT
ACTTATTTTTGCGCAGAAAAAGTAGTTTCTCGTGATCATAATTGGCTATATTTTGAGCG
TTCTGGTGCATGAACTAATAGTACCCTATGTGATCAGTTTCATTCAGAATCGTATGATC
GAATCGATGCAACTCTTCAATTCCATCTGCAATTCTTCATGGTTTTTGTCTACTGCTAT
GATTCTCTTCATGAATAAGAAGGTAATTAATCTCCCCCTGTGATCTAACTGTTCATGAG
TCTGTAACATATTAGCACAACACTATCCATCTCATTCCCATGTTTTCATCTCATTACAT
CTATCGTATCAATAATCTCTTCGTCTGGGAGATATCTCTCTTTCTCTGATATTTTTGGAC
TCTCTTCAAATCACACGATCTCTTGTCTAATCTTCGCTTCATTTGAGGATCTCTTCATG
GAGAAGATCAAACGCGTAAACATCACAACCGCCTTCCCTGATTATGAAGGTGAGAAG
GGAGAAGGGAAAGAGAAGGAAGACGGAGGGGGAGATGGGGGATCGACATCGATGG
ATTAAGTGGGCGATAATGCACTACAAGAGAATGACGTAGACTGATCATCATCAAGAT
TGAAATAACTGTTGTCTTTCCCGTAGACGATTATTCCCATCCCACACTATTTCTCATTT
GATCAAGTTCATTCTCAGGAGGGCAAAACTACGAAGAAGCAGTGAATTTCATCAAGC
AAAAGTTCGCTGAACTAAATATGAACCCTGATAAGAAGACGATTTACATGCACGAGA
CTTGCGCCACGGACACTAATCAGGTTAGACACATCGAGAGATTCGCAGTTATTTACTC
GCATTCCAAATGTTTGACTCTTGTGTAGTAGGCAACACACCACTTGCAATATCTATTG
AAGGAATATTGAACATTCTCCTAATTGCTTCGCTTGATTTTGTAATCAAAACACTTGTT
ATTCATAATTAAAACAAGCTGAATAGGATACCAATGAACACACTCACCTACCGTATCC
TTCAGGTTCAACTGGTCATTTCCTCTGTCATCGATACGATTATCCAGAAGAATCTACAG
AAGGCAGGAATGATGTAGACACTTCTCCTTTCTTCTTCAGAGGAATCCAATGATTTTTC
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AAACGAGCTTTTCAAACTCAATATTGATCGATATTTGCTCATTCACGTGTTCTCTTCTC
TTCTTATTCATCTCGTCCATAATATTTTGATCTCACTAATCGATAGTGTAGTCACGTCA
CTTACCGTACTACCGTTAGATCAAATTGAAATGTGATTTTGCTATATTTCGTGTACCCT
TACAAAACTTCGATCTCGATCCTTCCAAAAAGGAACAAATGTTCTCAATCCTCATGTA
TCCCTCCAACATTCTCCCCATTTGTCAAATGCATCTTTCTTTATAATGCATTTCGACCCA
TATCTCCATTCTCGATTGTTGTTTTTGCCTGCTAAATTCTGAATTCCCATCATAAATCTC
ACGTAGGTTTGATACTCTCGTCACATCGATTGCTGCGCATTCAGAGAAACCGAGTGAT
AAACAATTTTTATGAGATTTGGAGAGTGCGAAAAGAGTAACAATGAAATCAACAACG
AGAACAATCAATGCAGTTTGAGGTCTATTGTGGGACTAACTAACTGCTAACAATTAAT
GAGGGGAGGTTAGACCCGAAATGGAGTTGGGTGAGGAATTAGGCAGTTACTGATGAT
TAGGCGGTCTCCATAGATTCAATTACCTGAAATAAGAATGTCAATAATATAGAGATAT
ATACACTGATGCGATTGTACTTGTTCTATCACGATATTCCACAAGCTTCTTTTAGGAAA
GAGAAACTAGAGAAGAAATCACGCATTACATGAAAATATTGCAAACCTTATAGTAAG
GGCTGTTCTTGTATATTCTAATCTTCTTCTCGAGTTGCTTGGGAGTCTCTTGCCACAGA
TTGCGAGGATCGCGAGACATCGCCTGACATGTTAGAATTTTAGAAAACGATTATTTCC
ATAACCATAAATCCAGCATCAAATGTTACAGGAAGTTACATGTGAGAGGATAGTAAC
CTTAGTCGCCACCGTGGTTATTATCCTCATACATGTCTTTCATTTGAAATACATCTCAT
TTAAAAGACTGATAAGGTGACTTTTATAGTATTCGATTGTATGTAAATAAAATAACGA
ATAAATATAACCTTGTAGTCATCTTTGTGCTTCTCATACATGTATACGCAGAACTCGAT
ATCTCTCTCCTGGAGACGGAATTCGCGGCCAACCTGATAAAATCAACCAATGAAAAA
AAGACATTTTTGAAGGTAACAGATAGGACCGAATGA
6. How many exons does this coding region contain?
7. How many potential polyA consensus sequence ‘AATAAA’ sites are there?
8. You found a mutation in this gene (see below). Which part of the gene is the
mutation in (exon, intron, 5’ UTR, 3’ UTR)?
AGATGCCCTGCTCGCATTGTGGCAGGATAAAGGA_TGAAGAAAGCATATGAAA
TGAGGAGTGAATACCAACTCATCGACTCTGCTGCATTGTACGTTTCCCCTCTTC
ATACATCAAGATCTTCTATGATGCGTGCTATTCCCTCCCTATCACTGCAGCCTA
ATGAGTACTTCCCCTTCAGTTTCCTCGATAATGTTCAACGCATCGCTGAGCCTG
GATTCCGACCGACTGAGCAAGATATTCTCTTCTCGCGAGTGGCCACAACCGGA
GTCGTCGAAGTGAAATTCAAAATCAAGGAACTCGATTTCAG
9. How would this mutation affect the protein sequence?
Hong BIOL 360 Sp 2021
10. Using BamHI and PstI, what is the length of the odr-3 genomic DNA fragment will
be that will be cut? Can the fragment be cloned into the MCS of pUC19?