microbiology Gram Negative- unknown project.

jennie84
Descriptivechart73.xlsx

Sheet1

Descriptive Chart
Student Name:
Class Section:
Bacterium:
DNA sequence:
Morphological Characteristics
Gram reaction: negative
Cell shape: rod
Cell size: 0.4-0.8 x 1-3 um
Acid-fast: negative
Arrangement:
Endospores present: negative
Capsule present: negative
Motility: positive (swarming)
Cultural Characteristics
Optimum temperature: 37o C
Oxygen requirement: facultative anaerobe
Colony morphology on TSA plate: swarming with uniform film
Pattern of growth on TSA slant: filiform
Pattern of growth in TSB: flocculent, membrane surface
Type of hemolysis on BAP: gamma
Physiological Characteristics
Oxidation/Fermentation tests
Glucose (dextrose) fermentation: Acid & Gas
Lactose fermentation: negative
Mannitol fermentation: negative
Sucrose fermentation: Acid & Gas
Oxidation/Fermentation of glucose: O+/F+
Methyl Red (fermentation via mixed acid pathway): positive
Voges-Proskauer (fermentation via butanediol pathway: negative
Citrate utilization: negative (11-25% positive)
Nitrate reduction: positive
Oxidase test: negative
Hydrolytic and Degradative tests:
Catalase (breakdown of hydrogen peroxide): positive
Gelatin (gelatin hydrolysis): positive
Starch (amylase hydrolysis): negative
Skim milk (casein hydrolysis): negative
Urea (urea hydrolysis): positive
Indole (tryptophan hydrolysis): positive
Phenylalanine (phenylalanine deamination): positive
Other tests:
Hydrogen sulfide production: positive
DNase (DNA depolymerization): positive
Arginine (arginine decarboxylation): negative
Lysine (lysine decarboxylation): negative
Ornithine (ornithine decarboxylation): negative
Notes: