BIOLOGY

Experiment 1: Tissue Concentration

time. (secs.)

0.5 ml extract

1 ml. extract

2 ml extract

20 0.053 0.156 0.325 40 0.107 0.345 0.654 60 0.214 0.488 1.037 80 0.253 0.701 1.323 100 0.323 0.882 1.441 120 0.439 1.087 1.634

Experiment 2: Temperature

time. (secs.)

4˚ C 23˚ C 32˚ C 48˚ C

20 0.043 0.254 0.187 0.113 40 0.057 0.478 0.321 0.152 60 0.061 0.952 0.528 0.214 80 0.072 0.756 0.633 0.255 100 0.093 0.855 0.717 0.283 120 0.108 0.919 0.768 0.328

Experiment 3: pH

time. (secs.)

pH 3 pH 5 pH 7 pH 9

20 0.024 0.257 0.154 0.034 40 0.022 0.453 0.219 0.032 60 0.037 0.591 0.358 0.049 80 0.032 0.688 0.415 0.049 100 0.045 0.793 0.456 0.052 120 0.042 0.887 0.503 0.055

Experiment 4: Inhibitor

time. (secs.)

Control 1% Inhibitor 2.5% Inhibitor

5% Inhibitor

20 0.212 0.155 0.155 0.057 40 0.457 0.413 0.258 0.121 60 0.626 0.554 0.354 0.155 80 0.727 0.657 0.414 0.223 100 0.842 0.759 0.467 0.259 120 0.928 0.811 0.521 0.278

Experiment 5: Boiled Enzyme

time. (secs.)

Boiled Enzyme

20 0.027 40 0.013 60 0.021 80 0.015 100 0.029 120 0.022