Final Lab Report for Microbiology

ThaliaRamos99
ClassActivity7.docx

MCB3020L General Microbiology Laboratory

Lab #9:

Section: U01

Group: #5

Environment: Plant

Name: Thalia Ramos Gigato

ID: 6141550

Date: 03/22/2022

Lab Title: Isolation and Characterization of Bacteriophages.

CLASS ACITIVITY WORKSHEET

Note: Highlight those rows in which the obtained result does not match the expected results. Use superscript to indicate in-text citations for your results.

Test

Obtained Result

Expected Result

Insert name of bacterial species obtained from API system here: Proteus penneri

OF medium

With oil: +

Without oil: -

-1

Kligler’s Triple Iron Agar

+

+2

Litmus Milk

+

+2

Urease

+

+2

Starch Hydrolysis

+

+2

Nitrate Reduction

+

+2

Gelatin Hydrolysis

-

-2

Methyl Red

-

-2

Voges-Proskauer

-

+2

Oxidase

-

-1

Catalase

+

+1

Blood Agar

-

-2

MacConkey Agar

+

+2

EMB (Lactose)

-

-2

MSA (Mannitol)

Not Applicable

+2

List the 20 (or 50) names from your specific API test kit in the following rows.

ONPG

-

-2

DAH

+

+2

LDC

-

-2

ODC

-

-2

CIT

-

-2

H2S

-

-2

URE

+

+2

TDA

+

+2

IND

-

-2

VP

-

-2

GEL

-

-2

GLU

+

-2

MAN

-

-2

INO

-

-2

SOR

-

+2

RHA

-

+2

SAC

+

+2

MEL

-

+2

AMY

-

+2

ARA

+

+2

1Bergey’s Manual, 2nd Edition, 2004

2National Library of Medicine, 2016.

Question:

(A) Which results/tests were repeated in our experiments? List them.

The URE test was repeated, and it was positive for both.

(B) For each test, did the results match or not?

Some did match but 6 of them didn’t (highlighted ones).

1