Nutttttt
Calculation of Unknown Protein Sample Concentration
|
Tube |
absorbance reading at 562 nm |
average absorbance reading |
|
S1 (Blank) |
0.137 |
0.1435 |
|
S1(Blank) |
0.150 |
|
|
S2 |
0.698 |
0.698 |
|
S2 |
0.698 |
|
|
S3 |
1.156 |
1.167 |
|
S3 |
1.178 |
|
|
S4 |
2.016 |
2.046 |
|
S4 |
2.076 |
|
|
U1 |
2.004 |
2.0235 |
|
U1 |
2.043 |
|
|
U2 |
0.933 |
0.937 |
|
U2 |
0.941 |
|
|
U3 |
0.715 |
0.7185 |
|
U3 |
0.722 |
|
The standard curve was made by S1,S2,S3,S4(which have both concentration and absorbance). We add a trendline for this scatter plot, add the equation and R^2 for the trendline. R^2 is used to test if the model is fit or not. The closer to 1, the fitter model we get.
Now, we need to calculate the unknown samples (U1,U2,U3). We have U1, U2,U3’s absorbance. Now we need to calculate there concentration. The trendline will be used here.
Y=0.0019x+0.046
For U1, 2.0235=0.0019x+0.046
X=1040.789 ug/ml
For U2,
0.937=0.0019x+0.046
X=468.9474ug/ml
For U3,
0.7185=0.0019x+0.046
X= 353.9474ug/ml
Now we get the all the concentration of U1,U2 and U3.