Unknown project lab report
+ B + + + — + — — — — + — V — — + + B V + — + — + — – – + – – + +
henol Red TestsMorphologyRevised 11/98 Page 1A
Gram+ Bacilli
Microbe G
ra m
S ta
in
Sh ap
e
M ot
ili ty
E nd
os po
re s
C ap
su le
s
G lu
co se
(D ex
) B
la ck
C ap
L ac
to se
G re
en C
ap Su
cr os
e R
ed C
ap So
ri bi
to l
B lu
e C
ap M
an ni
to l
W hi
te C
ap D
ul ci
to l
P ur
pl e
C ap
In do
l
M et
hy l R
ed
V og
es Pr
os ka
ue r
C itr
at e
IMVIC
U re
as e
C oa
gu la
se
N itr
at e
R ed
uc tio
n
Biochemical Characteristics
St ar
ch H
yd ro
.
G el
at in
H yd
ro
O rn
ith in
e
Ph en
yl al
an in
e
C at
al as
e
O xi
da se
L itm
us M
ilk
T SI
H 2S
Sp ec
ia l N
ot es
+ B + + + — V — – + – + + + – – + +
+ B + + + V V — — + — + — + — — +
+ — — — + V + — + — — — + — — — — + NC
+ — — — + — + — — — — — — — — — — + NC
+ B — — — + + + — — — — — — — — — — — A —
Pleo
Pleo
+ Slow
+ (NO2)
+ (NO2
+ (NO2)
+ (NO2)
AS AB
+ (NO2)
+ (NO2)
+ (NO2)
Bacillus pumilus
Bacillus subtilis
Bacillus cereus
Bacillus megatherium
Corynebacterium bovis Corynebacterium xerosis Lactobacillus acidophilus
KEY + = 90% or more
+ in 24/48 hours (positive)
G = Gas
— = 90% or more — in 24/48 hours
V = 50% + or — in 24/48 hours (Variable)
= Test not run
C = Coccus B = Bacillus
Pleomorphic Granular Facultative 25°-35° to Dry, Light to None ± None — — — —
Coccobacilli Filiform Yellow Smooth
Pleomorphic Facultative 25°-35° to Filiform Off Smooth None ± None — — — —
Coccobacilli White
Singles Beige Facultative 25°-35° Very Large Irregular to Smooth None + Slight — ± — —
Bacilli White Beta
Beige Facultative 25°-35° Long Filamentous to Smooth None + Slight — ± — —
Chains Spreading White Beta
Singles Irregular Orange Granular som to Yellow t Bet ± — —
Short Chains Filiform Gold Yellow Smooth
Singles Facultative 25°-35° with Irregular Off Smooth None ± None — — — —
Pointed Ends White
M S
+ = Growth ± = Reduced Growth - = No Growth
O xy
ge n
R eq
ui re
m en
ts
O pt
im um
T em
pe ra
tu re
C el
l A
rr an
ge m
en t
C ol
on y
M or
ph ol
og y
Pi gm
en t
T ex
tu re
O do
r
E M
B
M A
C
SSPE A
H em
ol ys
is
General Information Cultural Characteristics TSA
Selective/Differential MediaRevised 11/98 Page 1B
Microbe
G+ Bacilli Bacillus
pumilus
Bacillus subtilis
Bacillus cereus
Bacillus megatherium
Corynebacterium bovis
Corynebacterium xerosis
Lactobacillus acidophilus
Chains Irregular Beige Granular Facultative 25°-35° some to to to None + Beta — ± — —
Singles Filiform White Smooth
Forms many Endospores
Forms few Endospores
Forms few Endospores
Forms few Endospores
Very Pleomorphic
Very Pleomorphic
Single Bacilli with Pointed End
Comments
+ C — — — + — + — — — — + — — — — —
+ C — — — + + + + + — + + — — — — — NC
+ C — — — — — — — — — — — — — +
+ C — — — + + + — — — — — — — — — — NC
Phenol Red TestsMorphologyRevised 11/98 Page 2A
Gram+ Cocci
Microbe G
ra m
S ta
in
Sh ap
e
M ot
ili ty
E nd
os po
re s
C ap
su le
s
G lu
co se
(D ex
) B
la ck
C ap
L ac
to se
G re
en C
ap Su
cr os
e R
ed C
ap So
ri bi
to l
B lu
e C
ap M
an ni
to l
W hi
te C
ap D
ul ci
to l
P ur
pl e
C ap
In do
l
M et
hy l R
ed
V og
es Pr
os ka
ue r
C itr
at e
IMVIC
U re
as e
C oa
gu la
se
N itr
at e
R ed
uc tio
n
Biochemical Characteristics
St ar
ch H
yd ro
.
G el
at in
H yd
ro
O rn
ith in
e
Ph en
yl al
an in
e
C at
al as
e
O xi
da se
L itm
us M
ilk
T SI
H 2S
Sp ec
ia l N
ot es
+ C — — ++ + V V V — — — — — — — — — NC
+ C — — — + + + + — + — — + — +
+ C — — — + + + — — + — — — — +
— (NO3) —
(NO3) —
(NO3) —
(NO3)
+ (NO2)
+ (NO2)
+ (NO2)
Streptococcus pneumoniae
Staphylococcus aureus Staphylococcus epidermidis Micrococcus luteus
+ C — — — + + — V — — — — — — — — — NC Streptococcus pyogenes
+ C — — — — — — — — — — — — — +
+ C — — — + — — — — — + — — — +
Sarcina lutea Gaffkya tetragena
A CW
Streptococcus mitis Streptococcus faecalis Streptococcus lactis
— (NO3)
— (NO3) —
(NO3)
L
Clusters Off White Facultative 25°-35° of Round to Smooth None + — ± (No Ferm) — — —
Cocci Beige Red
Clusters Off Yellow Slight Facultative 25°-35° of Round to Yellow- Smooth None + Beta to + (Ferm) ± — —
Cocci Mustard None Yellow
Short Small Facultative 30°-35° Chains of to None Smooth/ None + Alpha — — — —
Diplococci Medium Mucoid
Long Small Slight Facultative 25°-35° Chains of to None Smooth None + Alpha to — — — —
Cocci Pinpoint None Short Small
Facultative 25°-35° Chains of to None Smooth None + None — — — — Cocci Pinpoint
Long Small Facultative 25°-35° Chains of to None Smooth None + Alpha — — — —
Cocci Pinpoint
Clusters Aerobic 25°-30° of Round Beige Smooth None + — ± — — —
Cocci
M S
+ = Growth ± = Reduced Growth - = No Growth
O xy
ge n
R eq
ui re
m en
ts
O pt
im um
T em
pe ra
tu re
C el
l A
rr an
ge m
en t
C ol
on y
M or
ph ol
og y
Pi gm
en t
T ex
tu re
O do
r
E M
B
M A
C
SSPE A
H em
ol ys
is
General Information Cultural Characteristics TSA
Selective/Differential MediaRevised 11/98 Page 2B
Microbe
G+ Cocci
Streptococcus faccalis
Streptococcus lactis
Streptococcus pneumoniae
Staphylococcus aureus
Staphylococcus epidermidis
Micrococcus luteus
Inhibited by Optochin disk
Comments
Streptococcus mitis
Forms short chains with egg- shaped cells
Inhibited by Bacitracin disk
Long Small Facultative 30°-35° Chains of to None Smooth None + Beta — — — —
Cocci Pinpoint
Streptococcus pyogenes
Slow Growth
Clusters Creamy Aerobic 25°-30° with Packets Round to Bright Creamy None + — ± — — —
of 8 Cocci Convex Yellow Smooth
Sarcina lutea
Slow Growth
Packets Aerobic 25°-30° of Round Chalky Smooth None + — ± — — —
4 Cocci White
Gaffkya tetragina
Slow Growth
— C — — — — — — — — — — — — — — — — + + — C — — + + — + — — — — — — — — — — + +
Phenol Red TestsMorphologyRevised 11/98 Page 3A
Gram— Cocci
Microbe G
ra m
S ta
in
Sh ap
e
M ot
ili ty
E nd
os po
re s
C ap
su le
s
G lu
co se
(D ex
) B
la ck
C ap
L ac
to se
G re
en C
ap Su
cr os
e R
ed C
ap So
ri bi
to l
B lu
e C
ap M
an ni
to l
W hi
te C
ap D
ul ci
to l
P ur
pl e
C ap
In do
l
M et
hy l R
ed
V og
es Pr
os ka
ue r
C itr
at e
IMVIC
U re
as e
C oa
gu la
se
N itr
at e
R ed
uc tio
n
Biochemical Characteristics
St ar
ch H
yd ro
.
G el
at in
H yd
ro
O rn
ith in
e
Ph en
yl al
an in
e
C at
al as
e
O xi
da se
L itm
us M
ilk
T SI
H 2S
Sp ec
ia l N
ot es
— C — — — + — — — — — — — — — — — — + +
— + — — + — — + — — — — — + — — — + — —
— + — — + — — — + — — — — — + — — + — +
+ (NO2)
+ (NO2) —
(NO3)
— (NO3)
+ (NO2)
Rhodospirillum rubrum Aquaspirillum serpens
Neisseria sicca Branhamella catarrhalis Neisseria subflava
Spiral
Spiral KS BB
KS AB
G— Spirilli
+ + Aerobic 25°-30° Large Circular White Smooth None — — — Colorless Colorless —
Spirils Convex Lavendar Lt Yellow
Round Pink Aerobic 25°-30° Large to to Smooth None — — — — — —
Spirils Irregular Red
Round Aerobic 25°-30° Cocci to Grey- Granular None — — — — — —
in Pairs Irregular White
Aerobic 25°-30° Cocci Round Light Smooth None — — — — — — In Pairs Yellow
Aerobic 25°-30° Cocci Round Light Smooth None — — — — — — in Pairs Convex Yellow
M S
+ = Growth ± = Reduced Growth - = No Growth
O xy
ge n
R eq
ui re
m en
ts
O pt
im um
T em
pe ra
tu re
C el
l A
rr an
ge m
en t
C ol
on y
M or
ph ol
og y
Pi gm
en t
T ex
tu re
O do
r
E M
B
M A
C
SSPE A
H em
ol ys
is
General Information Cultural Characteristics TSA
Selective/Differential MediaRevised 11/98 Page 3B
Microbe
G- Cocci
Branhamella catarrhalis
Neisseria subflava
Rhodospirillum rubrum
Aquaspirillum serpens
Comments
Neisseria sicca
G- Spirilli
— B V — — — — — — — V + — — — — + +
— B + — — — — + + — — + — V — — — + — V
— B + — — — + — — — + + — V + — — — + +
— B + — — — — — — — — — — — + — — — — —
— B + — V — — — — — — — — — + — — — — — — B V — V — — — — — — — — — + — — — — V
K P K P
— — — — + — — + + — — — — — — — — — — —
— — — — + — — + + — — + — — — — — — — —
— B V — — + — + — — — — — — + — — — + — —
Phenol Red TestsMorphologyRevised 11/98 Page 4A
Gram— Bacilli
Microbe G
ra m
S ta
in
Sh ap
e
M ot
ili ty
E nd
os po
re s
C ap
su le
s
G lu
co se
(D ex
) B
la ck
C ap
L ac
to se
G re
en C
ap Su
cr os
e R
ed C
ap So
ri bi
to l
B lu
e C
ap M
an ni
to l
W hi
te C
ap D
ul ci
to l
P ur
pl e
C ap
In do
l
M et
hy l R
ed
V og
es Pr
os ka
ue r
C itr
at e
IMVIC
U re
as e
C oa
gu la
se
N itr
at e
R ed
uc tio
n
Biochemical Characteristics
St ar
ch H
yd ro
.
G el
at in
H yd
ro
O rn
ith in
e
Ph en
yl al
an in
e
C at
al as
e
O xi
da se
L itm
us M
ilk
T SI
H 2S
Sp ec
ia l N
ot es
— (NO3)
+ (N2) +
(NO2)
Alcaligenes faecalis Pseudomonas aeruginosa Pseudomonas fluorescens
+ (NO2) —
(NO2)
KS AB
Shigella boydii Shigella flexneri
B KS AB
+ (NO2)
+ (NO2) +slow (NO2)
Proteus vulgaris Morganella morgani
— B + — — — — + + — — + — + — — — + — ++
— (NO3)
+ (NO2)
+ (NO2)
Salmonella arizoniae
B
+ G
+ G
+ G +
G
sl+ V
KS NCB
KS NCB
KS NCB
KS BB
AS BB
KS AB
KS BB
sl+ V
Proteus mirabilis — B + — — — — — — — — + — + + — — + + ++
Salmonella pullorum
Salmonella typhimurium
+ ( G)
NC
KS (G) AB
KS (G) AB
| |
Singles, Flat, Offwhite, Dry to Foul + + colorless ± Aerobic 25°-35° Cocco- Spreading Greyish to Granular Sweaty — None — colorless transparent colorless
bacilli Irregular Grey pink to Smooth Socks translucent yellow
M S
+ = Growth ± = Reduced Growth - = No Growth
O xy
ge n
R eq
ui re
m en
ts
O pt
im um
T em
pe ra
tu re
C el
l A
rr an
ge m
en t
C ol
on y
M or
ph ol
og y
Pi gm
en t
T ex
tu re
O do
r
E M
B
M A
C
SSPE A
H em
ol ys
is
General Information Cultural Characteristics TSA
Selective/Differential MediaRevised 11/98 Page 4B
Microbe
Gram - Bacilli
Comments
Alcaligenes faecalis
Non Fermentor on EMB/ MAC/SS
Singles, Flat to raised Light Green Shiny Sweet + shiny ± shiny ± Aerobic 25°-35° Cocco- Irregular to Smooth Grapey — Slight — transparent transparent colorless
bacilli Spreading Blue Green Glistening Dirty Socks Beta to greenish to greenish to greenish
Pseudomonas aeruginosa
Non Fermentor on EMB/ MAC/SS
Singles, Raised Transparent Shiny Sweet + shiny + shiny ± shiny Aerobic 25°-35° Cocco- Irregular to Light Smooth Grapey — Slight — lavendar transparent transparent
bacilli Spreading Yellow Beta yellow yellow
Pseudomonas fluorescens
Non Fermentor on EMB/ MAC/SS
Swarms Smooth Foul + + colorless + colorless Facultative 25°-35° Singles, Rapid Translucent with Rotten — None — colorless transparent transparent
bacilli Spreader Ridges Egg to lavendar yellow yellow
Proteus mirabilis
Non Fermentor on EMB/ MAC/SS swarms
Swarm Smooth Foul + + colorless + colorless Facultative 25°-35° Singles, May Translucent with Rotten — None — colorless transparent transparent
bacilli Spread Ridges Egg to lavendar yellow yellow
Proteus vulgaris
+ + colorless + colorless Facultative 25°-35° Singles, Irregular Translucent Smooth Foul — None — colorless transparent transparent
bacilli Raised Spoiled to lavendar yellow yellow
Morganella morgani
Non Fermentor on EMB/ MAC/SS
Non Fermentor on EMB/ MAC/SS swarms
+ + colorless + colorless Facultative 25°-35° Singles, Round Translucent Smooth Slight — None — colorless transparent transparent
bacilli Raised Foul to lavendar yellow yellow
Salmonella pullorum
Non Fermentor on EMB/ MAC/SS
+ + colorless + colorless Facultative 25°-35° Singles, Round Translucent Smooth Slight — None — colorless transparent transparent
bacilli Raised Foul to lavendar yellow yellow
Salmonella arizoniae
Non Fermentor on EMB/ MAC/SS
+ + colorless + colorless Facultative 25°-35° Singles, Round Translucent Smooth Slight — None — colorless transparent yellowish
bacilli Raised Foul to lavendar yellow black center
Salmonella typhimurium
Non Fermentor on EMB/ MAC/SS
+ + colorless + colorless Facultative 25°-35° Singles, Round Translucent Smooth None — None — colorless transparent transparent
bacilli Raised to lavendar yellow yellow
Shigella boydii
Non Fermentor on EMB/ MAC/SS
+ + colorless + colorless Facultative 25°-35° Singles, Round Translucent Smooth None — None — colorless transparent transparent
bacilli Raised to lavendar yellow yellow
Shigella flexneri
Non Fermentor on EMB/ MAC/SS
— B V — V + + — + + — + — + — — — + — sl+
— B + — V + + + — — V + + — — — + — A —
— B V — V + — + + — + + — — — — — + — SLA —
Phenol Red TestsMorphologyRevised 11/98 Page 5A
Gram— Bacilli
Microbe G
ra m
S ta
in
Sh ap
e
M ot
ili ty
E nd
os po
re s
C ap
su le
s
G lu
co se
(D ex
) B
la ck
C ap
L ac
to se
G re
en C
ap Su
cr os
e R
ed C
ap So
ri bi
to l
B lu
e C
ap M
an ni
to l
W hi
te C
ap D
ul ci
to l
P ur
pl e
C ap
In do
l
M et
hy l R
ed
V og
es Pr
os ka
ue r
C itr
at e
IMVIC
U re
as e
C oa
gu la
se
N itr
at e
R ed
uc tio
n
Biochemical Characteristics
St ar
ch H
yd ro
.
G el
at in
H yd
ro
O rn
ith in
e
Ph en
yl al
an in
e
C at
al as
e
O xi
da se
L itm
us M
ilk
T SI
H 2S
Sp ec
ia l N
ot es
+ (NO2)
+ (NO2)
+ (NO2)
Escherichia coli Enterobacter aerogenes Klebsiella pneumoniae
AS AB
AS AB
AS AB
Citrobacter freundii Enterobacter cloacae
Serratia marcescans
+ G
+ (NO2) —
(NO3) +
(N2)
KS AB
AS BB
AS AB
+ (NO2)
KS BB
Edwardsiella tarda
+ G
+ G
+ G
SLA
SLA
G
G+
G
G
G
+ G
+ G
+ G
— B V — V V — — — — + + — — — — — + — +
— B V — ++ + + + + + — + — + — — — — — A —
— B V — + + + + + — — — + + — — — + — —
— B V — V + + + + — — — + + V — — + — A —
— SLA
SLA A|
Single Foul + light + ± Facultative 25°-35° bacilli Irregular Translucent Moist Dirty — — — purple to hot pink hot pink
some chains Raised Smooth Socks irridescent
M S
+ = Growth ± = Reduced Growth - = No Growth
O xy
ge n
R eq
ui re
m en
ts
O pt
im um
T em
pe ra
tu re
C el
l A
rr an
ge m
en t
C ol
on y
M or
ph ol
og y
Pi gm
en t
T ex
tu re
O do
r
E M
B
M A
C
SSPE A
H em
ol ys
is
General Information Cultural Characteristics TSA
Selective/Differential MediaRevised 11/98 Page 5B
Microbe
Gram - Bacilli
Comments
May form green sheen on EMB
Escherichia coli
Single Translucent + light + ± Facultative 25°-35° bacilli Round slightly Mucoid Slight to — — — purple to hot pink hot pink
thick capsules Convex creamy Slimy None pink
Enterobacter aerogenes
May form green sheen or “fish eye” colonies on EMB
Single Translucent + light + ± Facultative 25°-35° bacilli Round to White, Very mucoid Slight to — — — purple to hot pink hot pink
thick capsules Convex Creamy Slimy None pink
Klebsiella pneumoniae
May form green sheen on EMB very mucoid colonies
Red center + light + ± Facultative 25°-35° Tiny Irregular White Moist Slightly — — — purple to hot pink pinkish
coccobacilli Raised periphery Smooth Foul irridescent
May be colorless at higher temperatures
Serratia marcescans
Foul + light + ± Facultative 25°-35° Tiny Irregular Translucent Moist Dirty — — — purple to hot pink hot pink
coccobacilli Raised Smooth Socks irridescent
May form green sheen on EMB
Citrobacter freundii
Translucent Slight + light + ± Facultative 25°-35° Single Round Slightly Mucoid to — — — purple to hot pink pinkish
bacilli Convex Creamy Slimy None pink
May form mucoid colonies on EMB
Enterobacter cloacae
Slight + light + ± Facultative 25°-35° Single Irregular Translucent Moist to — — — purple to light pink pinkish
bacilli Raised Smooth None pink
Edwardsiella tarda